Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP47 antibody (GTX106253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using USP47(GTX106253) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX106253 diluted at 1:1000