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VASP (phospho Ser157) antibody

Anti-VASP (phospho Ser157) antibody used in Western Blot (WB). GTX32362
Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX32362

Cat. No. GTX32362

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all VASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of VASP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887653

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VASP (phospho Ser157) antibody used in Western Blot (WB). GTX32362

GTX32362 WB Image

WB analysis of PMA-treated C6 (A), HUVEC (B) whole cell lysates using GTX32362 VASP (phospho Ser157) antibody.

Anti-VASP (phospho Ser157) antibody used in IHC (Paraffin sections) (IHC-P). GTX32362

GTX32362 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32362 VASP (phospho Ser157) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Jayakumar T et al. Int J Mol Med 2017; 39 (1) : 174-182 Nobiletin, a citrus flavonoid, activates vasodilator-stimulated phosphoprotein in human platelets through non-cyclic nucleotide-related mechanisms.
Jayakumar T. et al. Int J Mol Med 2017; 39 : 174-182 Nobiletin, a citrus flavonoid, activates vasodilator-stimulated phosphoprotein in human platelets through non-cyclic nucleotide-related mechanisms.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399