Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC1 antibody [N1C2] (GTX114187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using VDAC1(GTX114187) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

VDAC1 antibody [N1C2] detects VDAC1 protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
VDAC1 antibody [N1C2] (GTX114187) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal PLoS One in 2015.PMID: 25658324