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VEGF antibody

Anti-VEGF antibody used in IHC (Paraffin sections) (IHC-P). GTX102643
Anti-VEGF antibody used in IHC (Paraffin sections) (IHC-P). GTX102643
Anti-VEGF antibody used in Western Blot (WB). GTX102643

Cat No. GTX102643

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2 conditioned medium , *A549

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VEGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174248

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular endothelial growth factor A , MVCD1 , VEGF , VPF

Cellular Localization

Secreted

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-VEGF antibody used in IHC (Paraffin sections) (IHC-P). GTX102643

GTX102643 IHC-P Image

VEGF antibody detects VEGF protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
VEGF antibody (GTX102643) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VEGF antibody used in IHC (Paraffin sections) (IHC-P). GTX102643

GTX102643 IHC-P Image

VEGF antibody detects VEGF protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
VEGF antibody (GTX102643) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VEGF antibody used in Western Blot (WB). GTX102643

GTX102643 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VEGF antibody (GTX102643) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

VEGF antibody Cat No. GTX102643

Rating ( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
ELISA(ELISA) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28604807
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Complement Altern Med in 2017.PMID: 28800782
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2017.PMID: 29104726
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019.PMID: 31430901
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ELISA
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019.PMID: 31430901
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf