VPAC2 antibody [HL3625] detects VPAC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
VPAC2 stained by VPAC2 antibody [HL3625] (GTX641600) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

VPAC2 antibody [HL3625] detects VPAC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VPAC2 stained by VPAC2 antibody [HL3625] (GTX641600) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

VPAC2 antibody [HL3625] detects VPAC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VPAC2 stained by VPAC2 antibody [HL3625] (GTX641600) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

VPAC2 antibody [HL3625] detects VPAC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse small intestine.
VPAC2 stained by VPAC2 antibody [HL3625] (GTX641600) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

VPAC2 antibody [HL3625] detects VPAC2 protein at suprachiasmatic nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VPAC2 stained by VPAC2 antibody [HL3625] (GTX641600) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled RMS 13 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.