Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108058 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VPS35 antibody detects VPS35 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
VPS35 antibody (GTX108058) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).