The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections.
PROPERTIES
Form
Liquid
Buffer
0.01M PBS pH7.4
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
von Willebrand Factor (Factor VIII-related antigen) from human plasma.
Purification
IgG fraction
Conjugation
Unconjugated
RRID
AB_369685
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
von Willebrand factor , F8VWF , VWD
Cellular Localization
Secreted
Background
Human von Willebrand factor (factor VIII R:Ag) is a 270kD multimeric plasma glycoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.
VWF antibody detects VWF protein at cell membrane in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. VWF antibody (GTX26994) diluted at 1:2000.
Show More +
GTX26994 IHC-P Image
IHC-P analysis of human kidney tissue using GTX26994 VWF antibody at 1:2,000.
Show More +
GTX26994 IHC-P Image
IHC-P analysis of human kidney tissue using GTX26994 VWF antibody at 1:2000.