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Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502]

Recombinant antibody Orthogonal
Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636976
Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636976
Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636976
Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Western Blot (WB). GTX636976
Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Western Blot (WB). GTX636976

Cat. No. GTX636976

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1502

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Venezuelan equine encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Venezuelan equine encephalitis virus nsp3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against Venezuelan Equine Encephalitis Virus nsP3, and it does not cross-react with nsP3 of Saint Louis encephalitis virus, La Crosse virus, Western Equine Encephalitis Virus, and Eastern Equine Encephalitis Virus.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Venezuelan equine encephalitis virus

Immunogen

Recombinant fragment of Venezuelan Equine Encephalitis Virus nsP3 (Strain P676).

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636976

GTX636976 ICC/IF Image

Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636976

GTX636976 ICC/IF Image

Orthogonal

Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976).
Sample: Multiple virus infected cells slide.
Green: Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636976

GTX636976 IHC-P (cell pellet) Image

Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and VEEV nsp3 (Venezuelan equine encephalitis virus) - transfected 293T cell pellet.
Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Western Blot (WB). GTX636976

GTX636976 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] used in Western Blot (WB). GTX636976

GTX636976 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations