Detection of VT1 by western blotting with anti -VT1 antibody 1. SDS-PAGE of culture medium of VTEC, stained with CBB 2. SDS-PAGE of crude extracts of VTEC cells, stained with CBB 3. Western blotting of culture medium of VTEC 4. Western blotting of crude extracts of VTEC cells. Ani-VT1 antibody was used at 1/2,000 dilution

Immunoprecipitation of VT1 from culture medium of VTEC with anti-VT1 antibody Arrows shows subunit A and subunit B of VT1. Heavy chain and Light chain indicate those of IgG