ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2.
Lane 1 : Culture medium of E. coli O157:H7
Lane 2 : Purified VT1
Lane 3 : Purified VT2
Lane 4 : VT non-producing E. coli

Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.