Villin antibody detects Villin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Villin stained by Villin antibody (GTX110034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX110034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Villin (GTX110034) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110034 diluted at 1:10000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody (GTX110034) diluted at 1:1000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed Hep3B, using Villin(GTX110034) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX110034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.