Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888145
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
vimentin
Background
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629743 WB Image
Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629743 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX629743 WB Image
Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis. A. 30 μg C8D30 whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg C2C12 whole cell lysate/extract 10% SDS-PAGE Vimentin antibody [GT7812] (GTX629743) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629743 IHC-Fr Image
Vimentin antibody [GT7812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. Blue: DAPI.
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GTX629743 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX629743 ELISA Image
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX629743 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Show More +
GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog intestine.Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629743 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Show More +
GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog colon.Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629743 ICC/IF Image
Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog spleen.Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629743 IHC-P Image
Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm in human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX629743 ICC/IF Image
Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX629743 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.