Vimentin antibody [GT7812]

Name: Vimentin antibody [GT7812]
Brand: GeneTex
SKU: GTX629743
Price: 329.00 USD
Rating: 4 (2 reviews)

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

Anti-Vimentin antibody [GT7812] used in IHC (Frozen sections) (IHC-Fr). GTX629743

Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743

Anti-Vimentin antibody [GT7812] used in ELISA (ELISA). GTX629743

Anti-Vimentin antibody [GT7812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629743

Cat No. GTX629743

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	GT7812
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629743, Detection: GTX100619/GTX112661

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , C8D30 , NIH-3T3 , C2C12 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NT2D1 , rat brain , *HEB , *A549 , *H358 , *MCF10A

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888145

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX629743 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629743 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629743 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX629743 WB Image

Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody [GT7812] (GTX629743) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629743 IHC-Fr Image

Vimentin antibody [GT7812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000.
Blue: DAPI.

GTX629743 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX629743 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629743 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX629743 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX629743 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629743 ICC/IF Image

Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX629743 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liang ZL et al. J Appl Toxicol 2018; (Epub) Epiproteome profiling of cadmium-transformed human bronchial epithelial cells by quantitative histone post-translational modification-enzyme-linked immunosorbent assay.

Application : WB

Reactivity : Human

Li SW et al. Sci Rep 2016; 6 : 25754 SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway.

Application : IHC-P

Reactivity : Mouse

Yang J et al. J Res Med Sci 2014; 19 (2) : 112-6 Adipose-derived stem cells from the breast.

Application : ICC/IF

Reactivity : Human

Submit a Reference

REVIEW