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Vimentin antibody [GT7812]

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in IHC (Frozen sections) (IHC-Fr). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743
Anti-Vimentin antibody [GT7812] used in ELISA (ELISA). GTX629743
Anti-Vimentin antibody [GT7812] used in ELISA (ELISA). GTX629743
Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743
Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743

Cat No. GTX629743

Host

Mouse

Clonality

Monoclonal

Clone Name

GT7812

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629743, Detection: GTX100619/GTX112661

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , C8D30 , NIH-3T3 , C2C12 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NT2D1 , rat brain , *HEB , *A549 , *H358 , *MCF10A

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888145

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody [GT7812] (GTX629743) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in IHC (Frozen sections) (IHC-Fr). GTX629743

GTX629743 IHC-Fr Image

Vimentin antibody [GT7812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000.
Blue: DAPI.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629743

GTX629743 ICC/IF Image

Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in Western Blot (WB). GTX629743

GTX629743 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Vimentin antibody [GT7812] used in ELISA (ELISA). GTX629743

GTX629743 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in ELISA (ELISA). GTX629743

GTX629743 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody [GT7812] used in IHC (Paraffin sections) (IHC-P). GTX629743

GTX629743 IHC-P Image

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Vimentin antibody [GT7812] Cat No. GTX629743

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF10A cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 01-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEB cells, A549 cells, H358 cells
Amount used : 30μg
Blocking : 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 16Hr
SDS
PBS.pdf