GeneTex
United States (US)

WGA Lectin (Biotin)

Cat No. GTX01501

Application WB, ELISA, IHC
PRODUCT

Product Description

Triticum vulgaris lectin against N-acetyl glucosamine (Biotin)
APPLICATION

Application Note

Histochemistry 1: 250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500. Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions. For Histochemistry the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied followed by streptavidin conjugated to enzyme and chromogen. For WB incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen. For ELISA the target proteins are absorbed on ELISA plate at a concentration of 5-10 μg/ml (50-100 μl), followed by blocking with protein solution, Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate. Please refer to Histochemistry, WB and ELISA protocol for detail information. The optimum dilution should be determined by the individual lab.

Specificity/Sensitivity

N-acetyl glucosamine (GlcNAc)
PROPERTIES

Form

Liquid

Buffer

10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide

Storage

Store at 2-8ºC.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wheat Germ agglutinin, Triticum vulgaris, Wheat germ agglutinin lectin, Wheat germ agglutinin, Triticum vulgaris, GlcNAc lectin, WGA, WGA Lectin, Triticum vulgaris lectin

Background

Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction. Inhibiting/Eluting sugars: 500 mM GlcNAc, dimer, trimmer or 100 mM acetic acid.Carbohydrate-Binding Specificity of WGA: Pentaacetylchitopentaose >Tetraacetylchitotetraose > triacetylchitotriose > Diacetylchitobiose > GlcNAc
Package List Price ($)
$ 89