Conjugation to Sepharose 4B (solid phase columns) to purify serum glycoproteins, membrane proteins and insulin receptors. For conjugation azide should be removed. Dilute in buffer containing 0.1 mM calcium chloride. The optimum dilution should be determined by the individual lab.
N-acetyl glucosamine (GlcNAc)
10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide
Store at 2-8ºC.
5 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Wheat Germ agglutinin, Triticum vulgaris, Wheat germ agglutinin lectin, Wheat germ agglutinin, Triticum vulgaris, GlcNAc lectin, WGA, WGA Lectin, Triticum vulgaris lectin
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction. Inhibiting/Eluting sugars: 500 mM GlcNAc, dimer, trimmer or 100 mM acetic acid.Carbohydrate-Binding Specificity of WGA: Pentaacetylchitopentaose >Tetraacetylchitotetraose > triacetylchitotriose > Diacetylchitobiose > GlcNAc