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West Nile Virus Core antibody

Anti-West Nile Virus Core antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85509
Anti-West Nile Virus Core antibody used in Western Blot (WB). GTX85509

Cat No. GTX85509

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity West Nile virus
Package
100 μg ($339)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
ELISA 1 μg/mL

Note :

ELISA
It will detect 10 ng of free peptide at 1 μg/mL.

Not tested in other applications.

Positive Control

THP-1 Cell Slide(GTX25540)

Specificity/Sensitivity

This antibody is specific for West Nile Virus Core C-Terminus
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

West Nile Virus Core antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the West Nile Virus core protein.The immunogen is located within amino acids 80 - 130 of West Nile Virus Core.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

West Nile Virus Core, WNV Core

Background

West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in 1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins (1) which play a major role for WNV entry into target cells (2,3). The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation (4).

Research Area

DATA IMAGES
Anti-West Nile Virus Core antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85509

GTX85509 ICC/IF Image

ICC/IF analysis of transfected Vero cells using GTX85509 West Nile Virus Core antibody.
Working concentration : 20 μg/ml

Anti-West Nile Virus Core antibody used in Western Blot (WB). GTX85509

GTX85509 WB Image

WB analysis of (A) untransfected or (B) transfected HeLa lysate using GTX85509 West Nile Virus Core antibody.
Working concentration : 1 μg/ml

REFERENCE
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REVIEW
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Package List Price ($)
$ 339