GeneTex

  • Country / Location Selection

United States (US)

Wnt1 antibody [10C8]

Anti-Wnt1 antibody [10C8] used in Western Blot (WB). GTX83320
Anti-Wnt1 antibody [10C8] used in IHC (Paraffin sections) (IHC-P). GTX83320
Anti-Wnt1 antibody [10C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83320
Anti-Wnt1 antibody [10C8] used in Flow cytometry (FACS). GTX83320

Cat. No. GTX83320

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10C8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Wnt1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of WNT1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 1 , BMND16 , INT1 , OI15

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt1 antibody [10C8] used in Western Blot (WB). GTX83320

GTX83320 WB Image

WB analysis of NIH3T3 (1), NIH3T3L1 (2) and HeLa (3) cell lysate using GTX83320 Wnt1 antibody [10C8].

Anti-Wnt1 antibody [10C8] used in IHC (Paraffin sections) (IHC-P). GTX83320

GTX83320 IHC-P Image

IHC-P analysis of human adrenal glands using GTX83320 Wnt1 antibody [10C8].

Anti-Wnt1 antibody [10C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83320

GTX83320 ICC/IF Image

ICC/IF analysis of HeLa (left) and 3T3-L1 (right) cells using GTX83320 Wnt1 antibody [10C8].
Green : Wnt1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Wnt1 antibody [10C8] used in Flow cytometry (FACS). GTX83320

GTX83320 FACS Image

FACS analysis of HeLa cells using GTX83320 Wnt1 antibody [10C8].
Green : Wnt1
Purple : negative control

REFERENCE

Application Reference

Vollersen N et al. Bone Res 2021; 9 (1) : 48 The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for Wnt1 antibody [10C8] (GTX83320). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399