Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt1 antibody (GTX105955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: WNT1 transfected 293T lysates
10% SDS PAGE
GTX105955 diluted at 1:5000

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt1 antibody (GTX105955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.