Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XPA antibody (GTX103168) diluted at 1:2000.

XPA antibody immunoprecipitates XPA protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of XPA protein by 2.5 μg of XPA antibody (GTX103168) 12% SDS-PAGE The immunoprecipitated XPA protein was detected by XPA antibody (GTX103168) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using XPA(GTX103168) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XPA(GTX103168) antibody at 1:200 dilution.