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YAP1 antibody [HL1894]

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643
Anti-YAP1 antibody [HL1894] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

Cat. No. GTX637643

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1894

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643

GTX637643 IHC-P Image

YAP1 antibody [HL1894] detects YAP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
YAP1 stained by YAP1 antibody [HL1894] (GTX637643) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody [HL1894] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637643

GTX637643 ICC/IF Image

YAP1 antibody [HL1894] detects YAP1 protein by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YAP1 stained by YAP1 antibody [HL1894] (GTX637643) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations