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YAP1 antibody [HL1894]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643
Anti-YAP1 antibody [HL1894] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637643
Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643
Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

Cat. No. GTX637643

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1894

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643

GTX637643 IHC-P Image

YAP1 antibody [HL1894] detects YAP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
YAP1 stained by YAP1 antibody [HL1894] (GTX637643) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody [HL1894] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637643

GTX637643 ICC/IF Image

YAP1 antibody [HL1894] detects YAP1 protein by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YAP1 stained by YAP1 antibody [HL1894] (GTX637643) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-YAP1 antibody [HL1894] used in IHC (Paraffin sections) (IHC-P). GTX637643

GTX637643 IHC-P Image

YAP1 antibody [HL1894] detects YAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
YAP1 stained by YAP1 antibody [HL1894] (GTX637643) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-YAP1 antibody [HL1894] used in Western Blot (WB). GTX637643

GTX637643 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations