Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

YTHDF2 antibody [HL2910] detects YTHDF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
YTHDF2 stained by YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

YTHDF2 antibody [HL2910] detects YTHDF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
YTHDF2 stained by YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

YTHDF2 antibody [HL2910] detects YTHDF2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YTHDF2 stained by YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL2910] (GTX640253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.