Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ZEB2 antibody detects ZEB2 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Mouse tissue extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ZEB2 antibody detects ZEB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Cancer Med in 2020.PMID: 32277605