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ZFAT antibody

Anti-ZFAT antibody used in IHC (Paraffin sections) (IHC-P). GTX122715
Anti-ZFAT antibody used in Western Blot (WB). GTX122715
Anti-ZFAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122715
Anti-ZFAT antibody used in IHC (Paraffin sections) (IHC-P). GTX122715

Cat. No. GTX122715

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZFAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZFAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and AT-hook domain containing , AITD3 , ZFAT1 , ZNF406

Cellular Localization

Nucleus , Cytoplasm , cytosol

Background

This gene encodes a protein that likely binds DNA and functions as a transcriptional regulator involved in apoptosis and cell survival. This gene resides in a susceptibility locus for autoimmune thyroid disease (AITD) on chromosome 8q24. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZFAT antibody used in IHC (Paraffin sections) (IHC-P). GTX122715

GTX122715 IHC-P Image

ZFAT antibody detects ZFAT protein at cytoplasm and nucleus in mouse placenta by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
ZFAT antibody (GTX122715) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZFAT antibody used in Western Blot (WB). GTX122715

GTX122715 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
5% SDS PAGE
GTX122715 diluted at 1:1000

Anti-ZFAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122715

GTX122715 ICC/IF Image

ZFAT antibody detects ZFAT protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZFAT protein stained by ZFAT antibody (GTX122715) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ZFAT antibody used in IHC (Paraffin sections) (IHC-P). GTX122715

GTX122715 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ZFAT(GTX122715) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169