WB analysis of various samples using GTX10550 ZIC2 antibody.
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Loading : 20 μg
Dilution : 1:500

Western blot using GeneTex's Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.