Zika virus NS4B protein antibody

Name: Zika virus NS4B protein antibody
Brand: GeneTex
SKU: GTX133321
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Zika virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133321

Anti-Zika virus NS4B protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133321

Anti-Zika virus NS4B protein antibody used in Western Blot (WB). GTX133321

Cat. No. GTX133321

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P (cell pellet)
Reactivity	Zika virus
Package	100 μl ($399), 25 μl ($169)

See all Zika virus NS4B protein products

View product citations for antibody GTX133321 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Zika virus NS4B-transfected 293T , Zika virus NS4B-infected C6/36

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Full length Zika virus NS4B recombinant protein. (Zika virus (strain H/PF/2013))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 4B , Zika virus NS4B , ZIKV Non-structural protein 4B , ZIKV NS4B

Research Area

DATA IMAGES

GTX133321 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B protein antibody (GTX133321).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green:Zika virus NS4B protein antibody (GTX133321) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX133321 IHC-P (cell pellet) Image

Zika virus NS4B protein antibody detects Zika virus NS4B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus NS4B transfected 293T.
Green: Zika virus NS4B stained by Zika virus NS4B protein antibody (GTX133321) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133321 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133321 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133321 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus NS4B protein antibodyantibody (GTX133321).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus NS4B protein antibodyantibody (GTX133321) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133321 WB Image

The data was published in the journal Elife in 2018.PMID: 30511641

GTX133321 WB Image

The data was published in the journal Elife in 2018.PMID: 30511641

GTX133321 WB Image

The data was published in the journal Elife in 2018.PMID: 30511641

REFERENCE

Application Reference

Laura Riva et al. J Virol 2021; 95 (22) : e0099621 The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment.

Application : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

Application : WB, ICC/IF

Rory K M Long et al. Sci Rep 2020; 10 (1) : 20937 Super resolution microscopy and deep learning identify Zika virus reorganization of the endoplasmic reticulum.

Application : ICC/IF

Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

Application : ICC/IF

Soto-Acosta R et al. Elife 2018; 7 Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA.

Application : WB

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