WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Loading : 10 ug per lane
Dilution : 1:500

WB analysis of various cell lines using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Loading : 35 ug per lane

IHC-P analysis of human kidney tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83663 alpha 1 Antitrypsin antibody [11G2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of human pancreas tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Loading : 5 ug per lane

IHC-P analysis of human liver tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.