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alpha Actinin 4 antibody [N2C1], Internal

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113115

Cat No. GTX113115

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

A431 , HepG2 , NIH-3T3 , mouse brain , PC-12

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949579

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus , Cytoplasm

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113115

GTX113115 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HepG2 (GTX27900)
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113115

GTX113115 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.

REFERENCE

There are currently no references for alpha Actinin 4 antibody [N2C1], Internal (GTX113115). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for alpha Actinin 4 antibody [N2C1], Internal (GTX113115). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169