APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HepG2 , NIH-3T3 , mouse brain , PC-12
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949579
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actinin alpha 4 , ACTININ-4 , FSGS , FSGS1
Cellular Localization
Nucleus , Cytoplasm
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113115 WB Image
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113115 WB Image
Sample (30 μg of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113115 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113115 WB Image
alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113115 WB Image
Sample (30 μg of whole cell lysate) A: A431 (GTX27909) B: HepG2 (GTX27900) 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113115 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for alpha Actinin 4 antibody [N2C1], Internal (GTX113115). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Actinin 4 antibody [N2C1], Internal (GTX113115). Be the first to share your experience with this product.