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alpha Actinin 4 antibody [N2C1], Internal

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115
Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

Cat. No. GTX113115

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Actinin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

Mouse brain , PC-12 , A431 , HepG2 , NIH-3T3

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

Cellular Localization

Nucleus , Cytoplasm

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Database

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

Research Area

DATA IMAGES

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113115

GTX113115 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113115

GTX113115 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX113115 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [N2C1], Internal used in Western Blot (WB). GTX113115

GTX113115 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [N2C1], Internal (GTX113115) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ilaria Grossi et al. Int J Mol Med 2021; 47 (2) : 533-546 MicroRNA?34a?5p expression in the plasma and in?its extracellular vesicle fractions in subjects with Parkinson's disease: An exploratory study.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169