IHC-P analysis of human lymphoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84661 alpha B Crystallin antibody [3C2].

IHC-P analysis of human pancreas carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human breast adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84661 alpha B Crystallin antibody [3C2].
Loading : 5 ug per lane

WB analysis of A549 and COS-7 cell lysate using GTX84661 alpha B Crystallin antibody [3C2].
Loading : 10 ug per lane
Dilution : 1:200

IHC-P analysis of human colon adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84661 alpha B Crystallin antibody [3C2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of human bladder carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.