GeneTex
United States (US)

alpha Cardiac Muscle Actin antibody

GTX101876_40800_20150306_WB_Merged_CT_19011506_434.jpg
GTX101876_39897_IHC_19011506_413.jpg
GTX101876_40800_IFA_19011506_251.jpg
GTX101876_39897_WB_R_19011506_763.jpg
GTX101876_41857_WB_19021719_483.jpg

Cat No. GTX101876

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NIH-3T3 , JC , BCL-1 , Rat muscle , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Cardiac Muscle Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Alpha, Cardiac Muscle 1,Actc,Asd5,Cmd1R,Cmh11,Lvnc4,Actc1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101876_40800_20150306_WB_Merged_CT_19011506_434.jpg

GTX101876 WB Image

alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) and Rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody (GTX101876) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101876_39897_IHC_19011506_413.jpg

GTX101876 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using alpha Actin (cardiac muscle)(GTX101876) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101876_40800_IFA_19011506_251.jpg

GTX101876 ICC/IF Image

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (GTX101876) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101876_39897_WB_R_19011506_763.jpg

GTX101876 WB Image

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha Actin (cardiac muscle) antibody (GTX101876) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101876_41857_WB_19021719_483.jpg

GTX101876 WB Image

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Actin (cardiac muscle) antibody (GTX101876) diluted by 1:500.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169