APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.24mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human alpha Synuclein Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MGC110988 antibody, NACP antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, SNCA antibody, non A-beta component of AD amyloid antibody, alpha-synuclein antibody, alpha-synuclein, isoform NACP140 antibody, synuclein alpha-140 antibody, synuclein, alpha (non A4 component of amyloid precursor) antibody
Cellular Localization
Cytoplasm , Membrane , Nucleus
Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 WB Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX112799 IHC-P Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
alpha Synuclein antibody (GTX112799) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112799 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112799 WB Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 IHC-P Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112799 ICC/IF Image
alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
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REFERENCE
