APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.74 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human alpha Synuclein Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618470
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
synuclein alpha , NACP , PARK1 , PARK4 , PD1
Cellular Localization
Cytoplasm , Membrane , Nucleus
Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX112799 IHC-P Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
alpha Synuclein antibody (GTX112799) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112799 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 IHC-P Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112799 ICC/IF Image
alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX112799 IHC-Fr Image
alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
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GTX112799 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112799 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX112799 IHC-P Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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![Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507](/upload/website/prouct_img/small/GTX101507/GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg "Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507")
