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alpha Synuclein antibody

Comparison
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112799
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

Cat No. GTX112799

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all alpha Synuclein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human alpha Synuclein Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
alpha Synuclein antibody (GTX112799) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112799

GTX112799 ICC/IF Image

alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zeng H et al. BMC Genomic 2019; 201 (1) : 851 Transcriptomic analysis of α-synuclein knockdown after T3 spinal cord injury in rats.
Application : WB
Reactivity : Rat
Han C et al. J Mol Med (Berl) 2019; (Epub) Exosomes from patients with Parkinson's disease are pathological in mice.
Application : WB, IHC-P
Reactivity : Human
Lee S et al. Korean J Physiol Pharmacol 2017; 21 (6) : 625-632 MPTP-induced vulnerability of * neurons in A53T α-synuclein overexpressed mice with the potential involvement of DJ-1 downregulation.
Application : WB
Reactivity : Mouse
Bastias-Candia S et al. Environ Toxicol 2015; 30 (6) : 656-63 Combined exposure to agriculture pesticides, paraquat and maneb, induces alterations in the N/OFQ-NOPr and PDYN/KOPr systems in rats: Relevance to sporadic Parkinson's disease.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169