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alpha Tubulin 1A antibody

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832
Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832
Anti-alpha Tubulin 1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109832
Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832
Anti-alpha Tubulin 1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109832
Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832
Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832
Anti-alpha Tubulin 1A antibody used in IHC (Paraffin sections) (IHC-P). GTX109832

Cat No. GTX109832

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , rat brain , Drosophila

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952426

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin alpha 1a , B-ALPHA-1 , LIS3 , TUBA3

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832

GTX109832 WB Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Raji whole cell extract
C. 30 μg 293T whole cell extract
D. 30 μg A431 whole cell extract
E. 30 μg HeLa whole cell extract
F. 30 μg HepG2 whole cell extract
G. 30 μg H1299 whole cell extract
H. 30 μg HCT116 whole cell extract
I. 30 μg MCF-7 whole cell extract
J. 30 μg NT2D1 whole cell extract
K. 30 μg PC-3 whole cell extract
L. 30 μg U87-MG whole cell extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832

GTX109832 WB Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg rat brain extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin 1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109832

GTX109832 ICC/IF Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin 1A stained by alpha Tubulin 1A antibody (GTX109832) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832

GTX109832 WB Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
B. 30 μg mouse brain extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin 1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109832

GTX109832 ICC/IF Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin 1A stained by alpha Tubulin 1A antibody (GTX109832) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832

GTX109832 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 1A antibody (GTX109832) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin 1A antibody used in Western Blot (WB). GTX109832

GTX109832 WB Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin 1A antibody used in IHC (Paraffin sections) (IHC-P). GTX109832

GTX109832 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A(GTX109832) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

alpha Tubulin 1A antibody Cat No. GTX109832

Rating ( Average 4 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 3 users reviews)
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse testis
Amount used : 20μg
Blocking : 5% milk/TBST, 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 12Hr
Date : Anonymous submitted on 07-May-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caki-1 cells
Amount used : 20μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Date : Anonymous submitted on 24-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 24Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf