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alpha Tubulin 4a antibody

Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX112653
Anti-alpha Tubulin 4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112653
Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX112653

Cat No. GTX112653

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin alpha 4a,ALS22,H2-ALPHA,TUBA1

Cellular Localization

Cytoplasm , Cytoskeleton , Microtubule

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX112653

GTX112653 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
alpha Tubulin 4a antibody
GTX112653 diluted at 1:1000

Anti-alpha Tubulin 4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112653

GTX112653 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin 4a(GTX112653) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX112653

GTX112653 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX112653 diluted at 1:3000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf