GeneTex
  • Country / Location Selection

United States (US)

alpha Tubulin 4a antibody

Anti-alpha Tubulin 4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113098
Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX113098
Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX113098
Anti-alpha Tubulin 4a antibody used in IHC (Paraffin sections) (IHC-P). GTX113098

Cat No. GTX113098

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 ,*mouse epididymis ,

Predict Reactivity

Rat, Sheep, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin alpha 4a,ALS22,H2-ALPHA,TUBA1

Cellular Localization

Cytoplasm , Cytoskeleton , Microtubule

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-alpha Tubulin 4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113098

GTX113098 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin 4a(GTX113098) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX113098

GTX113098 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX113098 diluted at 1:1000

Anti-alpha Tubulin 4a antibody used in Western Blot (WB). GTX113098

GTX113098 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: HeLa
D: Molt-4
10% SDS PAGE
GTX113098 diluted at 1:1000

Anti-alpha Tubulin 4a antibody used in IHC (Paraffin sections) (IHC-P). GTX113098

GTX113098 IHC-P Image

alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha Tubulin 4a antibody (GTX113098) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

alpha Tubulin 4a antibody Cat No. GTX113098

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 18-Apr-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : Epididymis tissue
Amount used : 10μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf