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alpha Tubulin antibody [B-5-1-2]

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304
Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304
Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

Cat No. GTX11304

Host

Mouse

Clonality

Monoclonal

Clone Name

B-5-1-2

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, African green monkey, Kangaroo rat, Sea Urchin, Chlamydomonas
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25-0.5 μg/ml
ICC/IF 0.5-1 μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

chicken fibroblast, FS11

Specificity/Sensitivity

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sea Urchin

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin alpha 4a , ALS22 , H2-ALPHA , TUBA1

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Database

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [B-5-1-2] at 0.5 μg/ml.

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

The WB analysis of alpha Tubulin antibody [B-5-1-2] was published by Auer TO and colleagues in the journal Elife in 2015 (PMID: 26076409)

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

The WB analysis of alpha Tubulin antibody [B-5-1-2] was published by Auer TO and colleagues in the journal Elife in 2015 (PMID: 26076409)

REFERENCE

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