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alpha Tubulin antibody [GT114]

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

Cat No. GTX628802

Host Mouse
Clonality Monoclonal
Clone Name GT114
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , zebrafish eye , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , *U87-MG

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, tubulin, alpha, ubiquitous antibody, tubulin alpha-1B chain antibody, tubulin, alpha 1b antibody

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) diluted at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX628802

GTX628802 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).
Blue: Nuclear staining with Hoechst 33342.

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 µg zebrafish eye lysate/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% methanol for 5 min.
Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Various whole cell extracts (15 | 5 | 2 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
F. 30 µg drosophila lysae/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
REVIEW

alpha Tubulin antibody [GT114] Cat No. GTX628802

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 08-Jun-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells
Amount used : 40 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, overnightHr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169