PRODUCT
Summary
alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , zebrafish eye , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , *U87-MG
Predict Reactivity
Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_2716636
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Research Area
DATA IMAGES
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GTX628802 ICC/IF Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX628802 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 IHC-Fr Image
Immunofluorescence photomicrographs of frozen sections of mouse brain. Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04). Blue: Nuclear staining with Hoechst 33342.
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628802 WB Image
alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628802 WB Image
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 μg zebrafish eye lysate/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 ICC/IF Image
TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX628802 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 WB Image
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg mouse brain lysate/extract D. 30 μg PC-12 whole cell lysate/extract E. 30 μg rat brain lysate/extract F. 30 μg drosophila lysae/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 ICC/IF Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX628802 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
alpha Tubulin antibody [GT114]
Cat No. GTX628802
Rating
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( Average 4.9 based on 21 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 20 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal ASN Neuro in 2013.
PMID: 24286475
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2020.
PMID: 32150830
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2020.
PMID: 32150830
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Dm
Figure Note :
The data was peer reviewed and published in the journal EMBO Mol Med in 2013.
PMID: 23828861
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2013.
PMID: 23977396
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Cell Mol Med in 2015.
PMID: 25683605
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Curr Biol in 2017.
PMID: 28966089
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 27307030
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2018.
PMID: 29933620
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal J Biol Chem in 2016.
PMID: 26742848
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal J Biol Chem in 2016.
PMID: 26742848
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Zfsh
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2014.
PMID: 25000307
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Cell Biosci in 2019.
PMID: 31044068
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2020.
PMID: 32005813
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Cell Mol Med in 2020.
PMID: 31821701
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Toxins (Basel) in 2019.
PMID: 31159487
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25811469
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25811469
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Cell Mol Med in 2020.
PMID: 31821701
Date :
Anonymous submitted on 08-Jun-2017
Application Tested :
WB
Sample Species :
Hu
Sample :
SW480 cells
Amount used :
40 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:20000, 4°C, 16Hr
Date :
Anonymous submitted on 24-Jun-2015
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
U87-MG cells
Blocking :
5% BSA in PBST, 25°C, 2Hr
Fixation :
4% PFA
Primary Antibody :
1:200, 4°C, 16Hr