alpha Tubulin antibody [GT114]

293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
F. 30 μg drosophila lysae/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).
Blue: Nuclear staining with Hoechst 33342.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

The data was published in the journal Nat Commun in 2016.PMID: 27221592

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701

The data was published in the journal PLoS One in 2015. PMID: 25811469

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701

The data was published in the journal PLoS One in 2015. PMID: 25811469

The data was published in the journal Nat Commun in 2020. PMID: 32005813

The data was published in the journal Toxins (Basel) in 2019. PMID: 31159487

The data was published in the journal Cell Biosci in 2019. PMID: 31044068

The data was published in the journal PLoS One in 2014. PMID: 25000307

The data was published in the journal Genome Biol in 2018.PMID: 29548335

The data was published in the journal PLoS Biol in 2019.PMID: 30883547

The data was published in the journal Nat Commun in 2019.PMID: 31278365

The data was published in the journal Physiol Rep in 2017.PMID: 28408640

The data was published in the journal PLoS One in 2017.PMID: 28467493

The data was published in the journal Biomolecules in 2020.PMID: 32635653

The data was published in the journal Genome Biol in 2018.PMID: 29548335

The data was published in the journal Biomolecules in 2020.PMID: 32635653

The data was published in the journal PLoS Biol in 2019.PMID: 30883547

The data was published in the journal Life Sci Alliance in 2019.PMID: 31767615

The data was published in the 2022 in Int J Mol Sci. PMID: 35055077

The data was published in the 2018 in Sci Rep. PMID: 30459446

The data was published in the 2022 in Int J Mol Sci. PMID: 35055077

The data was published in the 2018 in Sci Rep. PMID: 30459446