GeneTex
  • Country / Location Selection

United States (US)

alpha Tubulin antibody

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079

Cat No. GTX102079

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079

GTX102079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102079 diluted at 1:10000

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079

GTX102079 ICC/IF Image

alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

alpha Tubulin antibody Cat No. GTX102079

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary culture endothelial cells
Amount used : 5μg
Blocking : 3% BSA in TBST, 23°C, 2Hr
Primary Antibody : 1:2000, 25°C, 2Hr
SDS
Glycerol.pdf
Thimerosal.pdf