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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784

Cat No. GTX108784

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3

Predict Reactivity

Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX108784 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX108784) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784

GTX108784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin(GTX108784) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784

GTX108784 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B(GTX108784) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf