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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784

Cat. No. GTX108784

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

NIH-3T3 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952428

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX108784) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784

GTX108784 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B(GTX108784) antibody at 1:200 dilution.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX108784 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784

GTX108784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin(GTX108784) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Shimada M et al. Nutrition Research 2017; (Epub) Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Zuppinger C et al. EJH 2017; 61 : 2 Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes.
Spur EM et al. Toxicology 2016; 353-354 : 34-47 inhibition of chymotryptic-like standard proteasome activity exacerbates *-induced cytotoxicity in primary cardiomyocytes.
Application : WB
Reactivity : Mouse
Ho YC et al. Sci Rep 2016; 6 : 25374 A Novel Protective Function of 5-Methoxytryptophan in Vascular Injury.
Application : WB
Reactivity : Human
Jayachandran P et al. Neural Dev 2016; 11 (1) : 1 Microtubule-associated protein 1b is required for shaping the neural tube.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169