APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10724856
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Research Area
DATA IMAGES
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GTX110717 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Tubulin antibody (GTX110717) at 1:300 dilution.
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GTX110717 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110717 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX110717) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110717 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000.
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GTX110717 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Tubulin(GTX110717) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
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REFERENCE
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REVIEW
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