*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Mouse, Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b antibody"
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
GTX110717 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Tubulin(GTX110717) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
GTX110717 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000.
GTX110717 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX110717) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min