Immunofluorescence analysis of methanol-fixed HeLa, using beta actin(GTX124214) antibody at 1:500 dilution.

beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta actin antibody (GTX124214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta actin(GTX124214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX124214 diluted at 1:10000

Sample (30 ug of whole cell lysate)
A: Jurakt
B: Raji
C: K562
10% SDS PAGE
GTX124214 diluted at 1:10000

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.