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beta Catenin antibody [5H10]

Anti-beta Catenin antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34440
Anti-beta Catenin antibody [5H10] used in Western Blot (WB). GTX34440

Cat No. GTX34440

Host Mouse
Clonality Monoclonal
Clone Name 5H10
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Chicken

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

Boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

85 kDa. ( Note )


Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.




Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.


Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken beta-Catenin


Ab purified from Bioreactor Concentrate by Protein A/G




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Catenin Beta 1,Ctnnb,Evr7,Mrd19,Armadillo,Ctnnb1


The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]


Research Area

Anti-beta Catenin antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34440

GTX34440 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (5H10).

Anti-beta Catenin antibody [5H10] used in Western Blot (WB). GTX34440

GTX34440 WB Image

Western Blot of A431 and A549 Cell Lysates using Catenin, beta Mouse Monoclonal Antibody (5H10)

Package List Price ($)
$ 329