beta Catenin antibody [N1N2-2], N-term

Name: beta Catenin antibody [N1N2-2], N-term
Brand: GeneTex
SKU: GTX101435
Price: 329.00 USD
Rating: 4 (4 reviews)

Anti-beta Catenin antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101435

Anti-beta Catenin antibody [N1N2-2], N-term used in Western Blot (WB). GTX101435

Anti-beta Catenin antibody [N1N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101435

Anti-beta Catenin antibody [N1N2-2], N-term used in Immunoprecipitation (IP). GTX101435

Anti-beta Catenin antibody [N1N2-2], N-term used in Flow cytometry (FACS). GTX101435

Anti-beta Catenin antibody [N1N2-2], N-term used in ChIP assay (ChIP assay). GTX101435

Cat No. GTX101435

Reference 31

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
Reactivity	Human, Mouse, Rat, Rabbit
Package	100 μl ($329), 25 μl ($169)

See all beta Catenin products

PRODUCT

Summary

Beta-catenin antibody recognizes beta-catenin protein, which acts as a cell adhesion factor as well as an effector of Wnt signaling pathway-driven transcription. Mutation or dysregulation of beta-catenin is associated with many diseases, including developmental disorders and cancers. There are a plethora of commercially available beta-catenin antibodies, among which GeneTex’s beta-catenin antibody (GTX101435) is one of the most rigorously validated and widely cited for a number of applications.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:50-1:100
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HeLa , A549 , H1299 , HCT116 , PC-12 , Mouse brain , *human fibroblast , *LX2

Predict Reactivity

Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Database

Gene ID: 1499 CTNNB1
UniProt: P35222 CTNNB1

Research Area

DATA IMAGES

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 WB Image

Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101435 IHC-P Image

GTX101435 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435 WB Image

beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract |
7.5% SDS-PAGE
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 IP Image

Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody (GTX101435).
Western blot analysis was performed using beta Catenin antibody (GTX101435).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101435 FACS Image

beta Catenin antibody [N1N2-2], N-term (GTX101435) detects CTNNB1 protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101435 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101435 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

REFERENCE

Application Reference

Wang H et al. Br J Haematol 2020; 189 (6) : 1151-1164 Lycorine Targets Multiple Myeloma Stem Cell-Like Cells by Inhibition of Wnt/β-catenin Pathway.

Application : WB

Reactivity : Human

Fang CH et al. J Biomed Sci 2020; 27 (1) : 42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.

Application : WB

Reactivity : Human

M?nica G Mendoza-Rodr?guez et al. Int J Mol Sci 2020; 21 (6) Use of STAT6 Phosphorylation Inhibitor and Trimethylglycine as New Adjuvant Therapies for 5-Fluorouracil in Colitis-Associated Tumorigenesis.

Application : IHC-P

Reactivity : Mouse

Jiang X et al. J Stomatol Oral Maxillofac Surg 2020; (Epub) Bone regeneration and mineralisation was promoted during distraction osteogenesis by human periostin gene in rabbit mandibular model.

Application : WB

Reactivity : Rabbit

Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2) : 118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.

Application : WB

Reactivity : Human

Show More +

Submit a Reference

REVIEW

beta Catenin antibody [N1N2-2], N-term Cat No. GTX101435

Rating

( Average 4 based on 4 users reviews)

Application

Western Blot(WB)

( Average 4 based on 4 users reviews)

Date : Anonymous submitted on 01-Oct-2019

Score :

Application Tested : WB

Sample Species : Hu

Sample : Huh7 cells

Amount used : 5 μg

Blocking : 2.5% non-fat milk, 25°C, 0.5Hr

Primary Antibody : 1:2000, 25°C, 4Hr

Date : Anonymous submitted on 17-Jun-2016

Score :

Application Tested : WB

Sample Species : Hu

Sample : 293T cells

Amount used : 60μg

Blocking : 5% milk, 24°C, 1.5Hr

Primary Antibody : 1:1200, 4°C, 16Hr

Lane Description : Lane 1: 293T cells, Lane 2-3: knockdown of β Catenin in 293T cells

Date : Anonymous submitted on 28-May-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : LX2 cells

Amount used : 10μg

Blocking : 5% skim milk in TBST, 25°C, 1Hr

Primary Antibody : 1:500, 4°C, 16Hr

Lane Description : Lane 1: LX2 cells.Lane 2: LX2 cells (TGF-beta treatment).

Date : Anonymous submitted on 31-Mar-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : fibroblast cells

Amount used : 30μg

Blocking : 5% milk in TBST, 25°C, 1Hr

Primary Antibody : 1:2000, 4°C, 16Hr

Submit a Review

Application Reference

Wang H et al. Br J Haematol 2020; 189 (6):1151-1164 Lycorine Targets Multiple Myeloma Stem Cell-Like Cells by Inhibition of Wnt/β-catenin Pathway.

Application : WB

Reactivity : Human

Fang CH et al. J Biomed Sci 2020; 27 (1):42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.

Application : WB

Reactivity : Human

Application : IHC-P

Reactivity : Mouse

Jiang X et al. J Stomatol Oral Maxillofac Surg 2020; (Epub) Bone regeneration and mineralisation was promoted during distraction osteogenesis by human periostin gene in rabbit mandibular model.

Application : WB

Reactivity : Rabbit

Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2):118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.

Application : WB

Reactivity : Human

Callejas BE et al. Int J Cancer 2019; (Epub) Helminth-derived molecules inhibit colitis-associated colon cancer development through NF-庥B and STAT3 regulation.

Application : IHC-P

Reactivity : Mouse

Shen HH et al. J Biomed Sci 2019; 26 (1):62 Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3?3-L1 cells.

Application : WB

Reactivity : Rat

Fang Y et al. Nat Commun 2019; 10 (1):3981 CD36 inhibits 帣-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Application : IHC-P

Reactivity : Human

Lin JH et al. Cell Adh Migr 2019;:1-18 Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin.

Application : WB

Reactivity : Human

Jakubzig B et al. Cancers (Basel) 2018; 10 (12) Mechanisms of Matrix-Induced Chemoresistance of Breast Cancer Cells-Deciphering Novel Potential Targets for a Cell Sensitization.

Application : WB, FACS

Fang F et al. Oncotarget 2018; 9 (95):36780-36792 Targeting the Wnt/β-catenin pathway in human osteosarcoma cells.

Application : WB

Reactivity : Human

Leon-Cabrera S et al. Cancers (Basel) 2018; 10 (9) Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development.

Application : IHC-P

Reactivity : Mouse

Yan W et al. J Cell Physiol 2018; (Epub) Suppression of SEMA6C promotes preantral follicles atresia with decreased cell junctions in mice ovaries.

Application : WB

Reactivity : Mouse

Chen Z et al. Cancer Lett 2018; (Epub) LncTIC1 interacts with β-catenin to drive liver TIC self-renewal and liver tumorigenesis.

Application : WB

Reactivity : Human

Ogawa T et al. Oncotarget 2017; 8 (68):112550-112564 ST6GALNAC1 plays important roles in enhancing cancer stem phenotypes of colorectal cancer via the Akt pathway.

Piva MBR et al. Cancers (Basel) 2017; 9 (9) integrin activation contributes to lower * sensitivity in mv3 melanoma cells by inducing the wnt signalling pathway.

Application : FACS

Reactivity : Human

Yen CH et al. Journal of Ethnopharmacology 2017; (Epub) gynura divaricata attenuates tumor growth and tumor relapse after * therapy in hcc xenograft model through suppression of cancer stem cell growth and wnt.

Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.

Application : WB

Reactivity : Human

Lu J et al. EMBO Rep 2017; (Epub) The phosphorylation status of T522 modulates tissue-specific functions of SIRT1 in energy metabolism in mice.

Application : WB, IP

Reactivity : Mouse

Kung YA et al. Nucleic Acids Res 2016; Control of the negative IRES trans-acting factor KHSRP by ubiquitination.

Application : WB

Reactivity : Human

Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.

Application : WB

Reactivity : Human

Lee JG et al. J Biol Chem 2015; 290 (44):26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.

Application : WB

Reactivity : Human

Thiyagarajan V et al. PLoS One 2015; 10 (10):e0141285 Antroquinonol Targets FAK-Signaling Pathway Suppressed Cell Migration, Invasion, and Tumor Growth of C6 Glioma.

Application : WB

Reactivity : Rat

Chiang CY et al. Clin Cancer Res 2015; 21 (24):1352069 SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor.

Application : WB

Reactivity : Human

Huang RF et al. Int J Nanomedicine 2015; 10:2823-46 Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.

Application : WB

Reactivity : Human

Nakagawa H et al. Proc Natl Acad Sci U S A 2014; 111 (3):1090-5 Loss of liver E-cadherin induces sclerosing cholangitis and promotes carcinogenesis.

Application : WB

Reactivity : Human

Turato C et al. Br J Cancer 2014; 110 (11):2708-15 SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis.

Application : IHC-P

Reactivity : Human

Hsu TH et al. Cell Death Differ 2014; Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells.

Application : WB

Reactivity : Human

Lee JG et al. Mol Cell Biol 2014; IL-1β-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA.

Application : WB

Reactivity : Human

Tsai TH et al. PLoS One 2014; 9 (4):e95443 Pigment Epithelium-Derived Factor 34-mer Peptide Prevents Liver Fibrosis and Hepatic Stellate Cell Activation through Down-Regulation of the PDGF Receptor.

Application : WB

Reactivity : Rat

Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012:819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.

Application : WB

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 329
25 μl	$ 169
Qty

beta Catenin antibody [N1N2-2], N-term

Cat No. GTX101435

Host

Clonality

Isotype

Application

Reactivity

Summary

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX101435 IHC-P Image

GTX101435 WB Image

GTX101435 IHC-P Image

GTX101435 WB Image

GTX101435 IHC-P Image

GTX101435 WB Image

GTX101435 ICC/IF Image

GTX101435 IHC-P Image

GTX101435 IHC-P Image

GTX101435 IP Image

GTX101435 FACS Image

GTX101435 IHC-P Image

GTX101435 IHC-P Image

GTX101435 ICC/IF Image

GTX101435 IHC-P Image

GTX101435 IHC-P Image

GTX101435 WB Image

GTX101435 ICC/IF Image

GTX101435 WB Image

GTX101435 IHC-P Image

GTX101435 WB Image

GTX101435 IHC-P Image

GTX101435 IHC-P Image

GTX101435 ChIP assay Image

REFERENCE

Application Reference

REVIEW

beta Catenin antibody [N1N2-2], N-term Cat No. GTX101435

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IL1 beta antibody

PD-L1 antibody

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