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beta Catenin antibody [N1N2-2], N-term

KO/KD
GTX101435_42235_20151209_IHC-P_M_19011506_946.jpg
GTX101435_42235_20151209_IHC-P_M_2_19011506_175.jpg
GTX101435_42592_20161129_IHC-P_R_19011506_476.jpg
GTX101435_42536_20160830_IHC-P_M_19011506_903.jpg
GTX101435_39876_ChIP_19011506_649.jpg
GTX101435_39876_IHC_M_19011506_978.jpg
GTX101435_39876_WB_R_19011506_243.jpg
GTX101435_42669_20161228_IFA_19011506_422.jpg
GTX101435_42452_20160803_IHC-P_M_19011506_466.jpg
GTX101435_42452_20160713_IHC-P_H2_19011506_978.jpg
GTX101435_42235_20160205_IP_19011506_992.jpg
GTX101435_42235_20160715_FACS_19011506_591.jpg
GTX101435_39876_IHC_M_2_19011506_950.jpg
GTX101435_39876_20150206_IHC_M_3_19011506_736.jpg
GTX101435_42536_20160817_IFA_19011506_370.jpg
GTX101435_42564_20161129_IHC-P_M_19011506_618.jpg
GTX101435_42627_20161129_IHC-P_R_19011506_386.jpg
GTX101435_39876_WB_M_Brain_19011506_291.jpg
GTX101435_39876_IFA_19011506_155.jpg
GTX101435_42536_20161124_WB_19011506_374.jpg
GTX101435_42235_20160623_WB_shRNA_watermark_19011506_178.jpg
GTX101435_42452_20160713_IHC-P_H_19011506_996.jpg

Cat No. GTX101435

Reference 19
3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
Reactivity Human, Mouse, Rat
See all beta Catenin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:50-1:100
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HeLa , A549 , H1299 , HCT116 , PC-12 , Mouse brain , *human fibroblast , *LX2

Predict Reactivity

Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTNNB antibody, DKFZp686D02253 antibody, FLJ25606 antibody, FLJ37923 antibody, CTNNB1 antibody, catenin beta-1 antibody, catenin (cadherin-associated protein), beta 1, 88kDa antibody

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101435_42235_20151209_IHC-P_M_19011506_946.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42235_20151209_IHC-P_M_2_19011506_175.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42592_20161129_IHC-P_R_19011506_476.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42536_20160830_IHC-P_M_19011506_903.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_39876_ChIP_19011506_649.jpg

GTX101435 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.
GTX101435_39876_IHC_M_19011506_978.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101435_39876_WB_R_19011506_243.jpg

GTX101435 WB Image

beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract |
7.5% SDS-PAGE
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101435_42669_20161228_IFA_19011506_422.jpg

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX101435_42452_20160803_IHC-P_M_19011506_466.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42452_20160713_IHC-P_H2_19011506_978.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42235_20160205_IP_19011506_992.jpg

GTX101435 IP Image

Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody (GTX101435).
Western blot analysis was performed using beta Catenin antibody (GTX101435).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX101435_42235_20160715_FACS_19011506_591.jpg

GTX101435 FACS Image

beta Catenin antibody [N1N2-2], N-term (GTX101435) detects CTNNB1 protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
GTX101435_39876_IHC_M_2_19011506_950.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101435_39876_20150206_IHC_M_3_19011506_736.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101435_42536_20160817_IFA_19011506_370.jpg

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX101435_42564_20161129_IHC-P_M_19011506_618.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_42627_20161129_IHC-P_R_19011506_386.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101435_39876_WB_M_Brain_19011506_291.jpg

GTX101435 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101435 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101435_39876_IFA_19011506_155.jpg

GTX101435 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX101435_42536_20161124_WB_19011506_374.jpg

GTX101435 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000.
GTX101435_42235_20160623_WB_shRNA_watermark_19011506_178.jpg

GTX101435 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101435_42452_20160713_IHC-P_H_19011506_996.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
REFERENCE

Application Reference

Leon-Cabrera S et al. Cancers (Basel) 2018; 10 (9) Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development.
Application : IHC-P
Reactivity : Mouse
Yan W et al. J Cell Physiol 2018; (Epub ahead of print) Suppression of SEMA6C promotes preantral follicles atresia with decreased cell junctions in mice ovaries.
Application : WB
Reactivity : Mouse
Chen Z et al. Cancer Lett 2018; (Epub ahead of print) LncTIC1 interacts with β-catenin to drive liver TIC self-renewal and liver tumorigenesis.
Application : WB
Reactivity : Human
Piva MBR et al. Cancers (Basel) 2017; 9 (9) Integrin Activation Contributes to Lower Cisplatin Sensitivity in MV3 Melanoma Cells by Inducing the Wnt Signalling Pathway.
Application : FACS
Reactivity : Human
Yen CH et al. Journal of Ethnopharmacology 2017; (Epub ahead of print) Gynura divaricata attenuates tumor growth and tumor relapse after cisplatin therapy in HCC xenograft model through suppression of cancer stem cell growth and Wnt.
Show More +
Submit a Reference
REVIEW

beta Catenin antibody [N1N2-2], N-term Cat No. GTX101435

Rating
( Average 4 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4 based on 3 users reviews)
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Lane Description : Lane 1: 293T cells, Lane 2-3: knockdown of β Catenin in 293T cells
Date : Anonymous submitted on 28-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : LX2 cells
Amount used : 10μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : Lane 1: LX2 cells.Lane 2: LX2 cells (TGF-beta treatment).
Date : Anonymous submitted on 31-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : fibroblast cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Submit a Review
Application Reference
Leon-Cabrera S et al. Cancers (Basel) 2018; 10 (9) Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development.
Application : IHC-P
Reactivity : Mouse
Yan W et al. J Cell Physiol 2018; (Epub ahead of print) Suppression of SEMA6C promotes preantral follicles atresia with decreased cell junctions in mice ovaries.
Application : WB
Reactivity : Mouse
Chen Z et al. Cancer Lett 2018; (Epub ahead of print) LncTIC1 interacts with β-catenin to drive liver TIC self-renewal and liver tumorigenesis.
Application : WB
Reactivity : Human
Piva MBR et al. Cancers (Basel) 2017; 9 (9) Integrin Activation Contributes to Lower Cisplatin Sensitivity in MV3 Melanoma Cells by Inducing the Wnt Signalling Pathway.
Application : FACS
Reactivity : Human
Yen CH et al. Journal of Ethnopharmacology 2017; (Epub ahead of print) Gynura divaricata attenuates tumor growth and tumor relapse after cisplatin therapy in HCC xenograft model through suppression of cancer stem cell growth and Wnt.
Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Lu J et al. EMBO Rep 2017; (Epub ahead of print) The phosphorylation status of T522 modulates tissue-specific functions of SIRT1 in energy metabolism in mice.
Application : WB, IP
Reactivity : Mouse
Kung YA et al. Nucleic Acids Res 2016; Control of the negative IRES trans-acting factor KHSRP by ubiquitination.
Application : WB
Reactivity : Human
Li CH et al. Arch Toxicol 2016; (Epub ahead of print) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Lee JG et al. J Biol Chem 2015; 290 (44):26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.
Application : WB
Reactivity : Human
Thiyagarajan V et al. PLoS One 2015; 10 (10):e0141285 Antroquinonol Targets FAK-Signaling Pathway Suppressed Cell Migration, Invasion, and Tumor Growth of C6 Glioma.
Application : WB
Reactivity : Rat
Chiang CY et al. Clin Cancer Res 2015; 21 (24):1352069 SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor.
Application : WB
Reactivity : Human
Huang RF et al. Int J Nanomedicine 2015; 10:2823-46 Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.
Application : WB
Reactivity : Human
Nakagawa H et al. Proc Natl Acad Sci U S A 2014; 111 (3):1090-5 Loss of liver E-cadherin induces sclerosing cholangitis and promotes carcinogenesis.
Application : WB
Reactivity : Human
Turato C et al. Br J Cancer 2014; 110 (11):2708-15 SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis.
Application : IHC-P
Reactivity : Human
Hsu TH et al. Cell Death Differ 2014; Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells.
Application : WB
Reactivity : Human
Lee JG et al. Mol Cell Biol 2014; IL-1β-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA.
Application : WB
Reactivity : Human
Tsai TH et al. PLoS One 2014; 9 (4):e95443 Pigment Epithelium-Derived Factor 34-mer Peptide Prevents Liver Fibrosis and Hepatic Stellate Cell Activation through Down-Regulation of the PDGF Receptor.
Application : WB
Reactivity : Rat
Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012:819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Application : WB
Reactivity : Human
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