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beta Tubulin 2C antibody [C1C3]

Anti-beta Tubulin 2C antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115404
Anti-beta Tubulin 2C antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115404
Anti-beta Tubulin 2C antibody [C1C3] used in Western Blot (WB). GTX115404
Anti-beta Tubulin 2C antibody [C1C3] used in Western Blot (WB). GTX115404

Cat No. GTX115404

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin beta 4B class IVb,Beta2,LCAEOD,TUBB2,TUBB2C

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 2C antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115404

GTX115404 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using beta Tubulin 2C(GTX115404) antibody at 1:200 dilution.

Anti-beta Tubulin 2C antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115404

GTX115404 IHC-P Image

Beta Tubulin 2C antibody [C1C3] detects beta Tubulin 2C protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Beta Tubulin 2C antibody [C1C3] (GTX115404) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin 2C antibody [C1C3] used in Western Blot (WB). GTX115404

GTX115404 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115404 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin 2C antibody [C1C3] used in Western Blot (WB). GTX115404

GTX115404 WB Image

Sample (30 µg of whole cell lysate)
A: A431
10% SDS PAGE
GTX115404 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169