beta Tubulin 3/ Tuj1 antibody [2E9]

Cat. No. GTX83356

Host	Mouse
Clonality	Monoclonal
Clone Name	2E9
Isotype	IgG1
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX83356 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human TUBB3 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Gene ID: 10381 TUBB3
UniProt: Q13509 TUBB3

Research Area

DATA IMAGES

GTX83356 ICC/IF Image

ICC/IF analysis of PANC-1 cells using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].
Green : beta Tubulin 3/ Tuj1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX83356 WB Image

WB analysis of HepG2 (1), A549 (2) and HeLa (3) cell lysate using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].

GTX83356 FACS Image

FACS analysis of A549 cells using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].
Green : beta Tubulin 3/ Tuj1
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

beta Tubulin 3/ Tuj1 antibody [2E9]

Cat. No. GTX83356

Host

Clonality

Clone Name

Isotype

Application

Reactivity