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beta Tubulin 3/ Tuj1 antibody [2E9]

Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83356
Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Flow cytometry (FACS). GTX83356
Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Western Blot (WB). GTX83356

Cat No. GTX83356

Host Mouse
Clonality Monoclonal
Clone Name 2E9
Isotype IgG1
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human TUBB3 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta 3 Class Iii,Cdcbm,Cdcbm1,Cfeom3,Cfeom3A,Feom3,Tubb4,Beta-4,Tubb3

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83356

GTX83356 ICC/IF Image

ICC/IF analysis of PANC-1 cells using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].
Green : beta Tubulin 3/ Tuj1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Flow cytometry (FACS). GTX83356

GTX83356 FACS Image

FACS analysis of A549 cells using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].
Green : beta Tubulin 3/ Tuj1
Purple : negative control

Anti-beta Tubulin 3/ Tuj1 antibody [2E9] used in Western Blot (WB). GTX83356

GTX83356 WB Image

WB analysis of HepG2 (1), A549 (2) and HeLa (3) cell lysate using GTX83356 beta Tubulin 3/ Tuj1 antibody [2E9].

REFERENCE
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REVIEW
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Package List Price ($)
$ 329