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beta Tubulin 3/ Tuj1 antibody [TU-20]

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

Cat No. GTX27751

Host

Mouse

Clonality

Monoclonal

Clone Name

TU-20

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

PROPERTIES

Form

Liquid

Buffer

PBS, 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Purification

Purified by precipitation and chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDCBM,CDCBM1,CFEOM3,CFEOM3A,FEOM3,TUBB3,TUBB4,beta4,tubulin beta 3 class III,Beta III tubulin,Tuj1,beta tubulin 3,Tuj-1

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

ICC/IF analysis of P-19 cells stimulated to neuronal differentiation by retinoic acid using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20] and GTX27792 beta Tubulin antibody [TU-06].
Panel A : GTX27751 (Red)
Panel B : GTX27751 (Red) and GTX27792 (Green)

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

ICC/IF analysis of Neuro2a cells using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20].
Green : Primary antibody
Blue : DAPI
Diultion : 3μg/ml

REFERENCE

REVIEW

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Package List Price ($)
$ 319