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beta Tubulin 3/ Tuj1 antibody [TU-20]

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

Cat. No. GTX27751

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TU-20

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
Package
100 μg ($399)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Purification

Purified by precipitation and chromatography

Conjugation

Unconjugated

RRID

AB_385733

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB3 , TUBB4 , beta4 , tubulin beta 3 class III , Beta III tubulin , Tuj1 , beta tubulin 3 , Tuj-1

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

ICC/IF analysis of Neuro2a cells using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20].
Green : Primary antibody
Blue : DAPI
Diultion : 3μg/ml

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

ICC/IF analysis of P-19 cells stimulated to neuronal differentiation by retinoic acid using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20] and GTX27792 beta Tubulin antibody [TU-06].
Panel A : GTX27751 (Red)
Panel B : GTX27751 (Red) and GTX27792 (Green)

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

The data was published in the journal PLoS One in 2012.PMID: 23071813

REFERENCE

Application Reference

Schaefers C et al. Stem Cells Int 2022; 2022 : 1320950 The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.
Application : ICC/IF
Reactivity : Human
Liu KC et al. Oncotarget 2013; 4 (12) : 2366-82 Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.
Reactivity : Human
Lehmann M et al. PLoS One 2013; 8 (8) : e70913 Evidence for a critical role of catecholamines for cardiomyocyte lineage commitment in murine embryonic stem cells.
Application : IHC
Reactivity : Mouse
Park JH et al. J Korean Neurosurg Soc 2012; 52 (3) : 246-9 Intracranial extraskeletal myxoid chondrosarcoma : case report and literature review.
Application : IHC
Reactivity : Human
Chien HC et al. PLoS One 2012; 7 (10) : e47481 Targeted disruption in mice of a neural stem cell-maintaining, KRAB-Zn finger-encoding gene that has rapidly evolved in the human lineage.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399