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beta Tubulin 3/ Tuj1 antibody

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129913
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX129913
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129913
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX129913

Cat No. GTX129913

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , SK-N-SH , mouse brain , rat brain

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.167 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Tubulin 3/ TUJ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin beta 3 class III,CDCBM,CDCBM1,CFEOM3,CFEOM3A,FEOM3,TUBB4,beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129913

GTX129913 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX129913

GTX129913 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at a dilution of 1:10000.

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129913

GTX129913 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody (GTX129913) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX129913

GTX129913 WB Image

Various tissue extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody (GTX129913) diluted at 1:10000.

REFERENCE
There are currently no references for beta Tubulin 3/ Tuj1 antibody (GTX129913). Be the first to share your publications with this product.
REVIEW

beta Tubulin 3/ Tuj1 antibody Cat No. GTX129913

Rating ( Average 3 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 16-Nov-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Dental pulp stem cells
Blocking : 5% NGS in 0.1% Triton-X in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.1% Triton-X in PBS
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169