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beta Tubulin 3/ Tuj1 antibody

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunoprecipitation (IP). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130245

Cat No. GTX130245

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human beta Tubulin 3/ TUJ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin beta 3 class III,CDCBM,CDCBM1,CFEOM3,CFEOM3A,FEOM3,TUBB4,beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130245

GTX130245 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at a dilution of 1:1000.

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunoprecipitation (IP). GTX130245

GTX130245 IP Image

Immunoprecipitation of beta Tubulin 3/ TUJ1 protein from SK-N-SH whole cell extracts using 5 μg of beta Tubulin 3/ TUJ1 antibody (GTX130245).
Western blot analysis was performed using beta Tubulin 3/ TUJ1 antibody (GTX130245).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130245

GTX130245 IHC-P Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:50000.

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130245

GTX130245 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW

beta Tubulin 3/ Tuj1 antibody Cat No. GTX130245

Rating ( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-Mar-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Neurons differentiated from neural stem cells
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 26-Mar-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Cerebrum tissue (E14.5)
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169