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beta Tubulin 3/ Tuj1 antibody

KO/KD
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunoprecipitation (IP). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130245

Cat. No. GTX130245

Reference 11
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain , HeLa

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human beta Tubulin 3/ TUJ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886220

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130245

GTX130245 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130245

GTX130245 IHC-P Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:50000.

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

KO/KD

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunoprecipitation (IP). GTX130245

GTX130245 IP Image

Immunoprecipitation of beta Tubulin 3/ TUJ1 protein from SK-N-SH whole cell extracts using 5 μg of beta Tubulin 3/ TUJ1 antibody (GTX130245).
Western blot analysis was performed using beta Tubulin 3/ TUJ1 antibody (GTX130245).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX130245

GTX130245 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at a dilution of 1:1000.

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130245

GTX130245 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

The staining pattern is based on the publication:PMID: 30390091

REFERENCE

Application Reference

Tian X et al. Mol Cell Proteomics 2024; 23 (2) : 100723 Multi-Omics Profiling Identifies Microglial Annexin A2 as a Key Mediator of NF-κB Pro-inflammatory Signaling in Ischemic Reperfusion Injury.
Yang JL et al. Neural Regen Res 2023; 18 (7) : 1521-1526 Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes.
Application : IHC-Fr
Reactivity : Mouse
Windster JD et al. EMBO Rep 2023; 24 (4) : e55789 A combinatorial panel for flow cytometry-based isolation of enteric nervous system cells from human intestine.
Application : FACS
Reactivity : Human
Shi Y et al. J Neuroinflammation 2023; 20 (1) : 91 N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1.
Application : IHC-Fr
Reactivity : Mouse
Fang C et al. Neural Regen Res 2023; 18 (4) : 895-900 Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in rats.
Application : WB, IHC-P
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

beta Tubulin 3/ Tuj1 antibody Cat. No. GTX130245

Rating
( Average 4 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130245
Date : Anonymous submitted on 09-Dec-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human colon tissue
Blocking : Ventana inhibitor CM, 5 min, 37°C
Fixation : 10% neutral buffered formalin
Primary Antibody : 1:300, 37°C, 0.33Hr
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130245
Date : Anonymous submitted on 26-Mar-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Neurons differentiated from neural stem cells
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130245
Date : Anonymous submitted on 26-Mar-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Cerebrum tissue (E14.5)
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Tian X et al. Mol Cell Proteomics 2024; 23 (2):100723 Multi-Omics Profiling Identifies Microglial Annexin A2 as a Key Mediator of NF-κB Pro-inflammatory Signaling in Ischemic Reperfusion Injury.
Yang JL et al. Neural Regen Res 2023; 18 (7):1521-1526 Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes.
Application : IHC-Fr
Reactivity : Mouse
Windster JD et al. EMBO Rep 2023; 24 (4):e55789 A combinatorial panel for flow cytometry-based isolation of enteric nervous system cells from human intestine.
Application : FACS
Reactivity : Human
Shi Y et al. J Neuroinflammation 2023; 20 (1):91 N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1.
Application : IHC-Fr
Reactivity : Mouse
Fang C et al. Neural Regen Res 2023; 18 (4):895-900 Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in rats.
Application : WB, IHC-P
Reactivity : Rat
Lingling Chen et al. J Nanobiotechnology 2022; 20 (1):210 Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury.
Application : WB, IHC-P
Reactivity : Mouse
Li S et al. Exp Eye Res 2021; 215:108900 Tauopathy induces degeneration and impairs regeneration of sensory nerves in the cornea.
Application : ICC/IF
Reactivity : Mouse
Montnach J et al. Br J Pharmacol 2021; 178 (13):2632-2650 Fluorescent- and tagged-protoxin II peptides: potent markers of the Nav 1.7 channel pain target.
Yang Y et al. Biomaterials 2021; 269:120479 Small molecules combined with collagen hydrogel direct neurogenesis and migration of neural stem cells after spinal cord injury.
Application : ICC/IF
Reactivity : Mouse
Ma D et al. Biomaterials 2020; (Epub) A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation.
Chen C et al. Biochim Biophys Acta 2017; 1864 (10):1900-1912 Critical role of TRPC1 in thyroid hormone-dependent dopaminergic neuron development.
Application : ICC/IF
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169