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beta Tubulin 3/ Tuj1 antibody

Orthogonal
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX50789
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50789
Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50789

Cat. No. GTX50789

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.442~446(E-E-S-E-A) derived from human beta Tubulin 3/ Tuj1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX50789

GTX50789 WB Image

WB analysis of extracts from mouse brain tissue, HeLa, JK, and P19 cells using GTX50789 beta Tubulin 3/ Tuj1 antibody.

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50789

GTX50789 IHC-Fr Image

Orthogonal

The data was published in the journal Stem Cells Int in 2019. PMID: 31531025

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50789

GTX50789 IHC-Fr Image

Orthogonal

The data was published in the journal Stem Cells Int in 2019. PMID: 31531025

REFERENCE

Application Reference

Paes-Colli Y et al. Front Cell Neurosci 2023; 17 : 1134130 Activation of cannabinoid type 1 receptor (CB1) modulates oligodendroglial process branching complexity in rat hippocampal cultures stimulated by olfactory ensheathing glia-conditioned medium.
Evaristo-Mendon?a F et al. Stem Cells Int 2019; 2019 : 7692973 Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists.
Application : ICC/IF
Reactivity : Mouse
Fabiana Evaristo-Mendon?a et al. Stem Cells International 2019; 2019 : Article ID 7692973 Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists.
Application : ICC/IF
Reactivity : Rat
Chen C et al. Stem Cells Dev 2015; Thyroid Hormone-Otx2 Signaling Is Required for Embryonic Ventral Midbrain Neural Stem Cells Differentiated into * Neurons.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399