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beta Tubulin 3/ Tuj1 antibody

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

Cat No. GTX85469

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat
Package
50 μg ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000-1:20000
ICC/IF Assay dependent
IHC 1:2000-1:5000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

tubulin, beta 3
PROPERTIES

Form

Liquid

Buffer

PBS + sodium azide (0.02%, w/v)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta 3 Class Iii,Cdcbm,Cdcbm1,Cfeom3,Cfeom3A,Feom3,Tubb4,Beta-4,Tubb3

Cellular Localization

Cytoplasmic

Background

Human beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) neonatal mouse brain cultures using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : GFAP antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469

GTX85469 WB Image

WB analysis of adult mouse brain lysate using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Dilution : 1:3000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

REFERENCE
REVIEW
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Package List Price ($)
$ 289