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beta Tubulin I antibody [SAP.4G5]

Anti-beta Tubulin I antibody [SAP.4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11312
Anti-beta Tubulin I antibody [SAP.4G5] used in Western Blot (WB). GTX11312

Cat No. GTX11312

Host Mouse
Clonality Monoclonal
Clone Name SAP.4G5
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse, Rat, Bovine, Dog, Hamster, Chicken, Xenopus
Package
100 μl ($389)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:20,000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent

Note :

WB
using methanol/acetone-fixed chicken fibroblasts

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

chicken fibroblast

Specificity/Sensitivity

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta Class I,Cdcbm6,Cscsc1,M40,Ok/Sw-Cl.56,Tubb1,Tubb5,Tubb

Background

The major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, betaI is constitutive and found in most tissues.

Database

Research Area

DATA IMAGES
Anti-beta Tubulin I antibody [SAP.4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11312

GTX11312 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

Anti-beta Tubulin I antibody [SAP.4G5] used in Western Blot (WB). GTX11312

GTX11312 WB Image

WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000.

REFERENCE
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REVIEW
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Package List Price ($)
$ 389